@conference {42018,
	title = {Regulation of milk protein synthesis in the bovine mammary gland : A proteomic approach},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {67},
	year = {2007},
	month = {Jan},
	pages = {407-412},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	address = {Wanaka},
	abstract = {The molecular mechanisms that regulate milk protein synthesis in the dairy cow are not well understood. We used a proteomic approach to identify potential regulators of milk protein synthesis among the endoplasmic reticulum (ER) proteins. Mammary tissue was collected from mid-lactation dairy cows treated with atropine (Atr; decreased milk production), growthhormone (GH; increased milk production) or vehicle alone. Fractions enriched for ER associated proteins were prepared by differential centrifugation and then resolved by 2-dimensional electrophoresis (2-DE). Proteins were visualised using colloidal coomassie G-250 and the phosphor-protein specific stain, Pro-Q Diamond. We identified 4 significant (P<0.05) and 9 marginally significant (P<0.15) spots that were differentially expressed between treatment groups. Six spots were subjected to LTQ ion trap tandem mass spectrometry and 2 proteins were identified with a high level of confidence: major vault protein (MVP) (27\% of the control level after GH treatment, P<0.05), and heat shock 70Kda protein 8 (HSPA8) (41\% of the control level after Atr treatment, P <0.01). Western blotting showed a 24\% decrease in HSPA8 following Atr treatment (P<0.10) and a 55\% decrease in MVP following GH treatment (P<0.10). These results provide a focus for further investigation of the role of MVP and HSPA8 in influencing milk production.},
	issn = {1176-5283},
	author = {Smolenski G and Bond JJ and Wheeler TT and Roy NC and McNabb WC and McCoard SA}
}
