@conference {41433,
	title = {Lack of genetic association of markers near the leptin gene with carcass fat content in Coopworth sheep lines selected for and against fatness},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {63},
	year = {2003},
	month = {Jan},
	pages = {176-178},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	address = {Queenstown},
	abstract = {Leptin is an important hormone in controlling food intake and energy expenditure in mammalian species. The present study evaluated levels of serum leptin in Coopworth sheep selected for and against subcutaneous fat depth for 10 generations. Significant differences in serum leptin concentrations were found between fat and lean sheep (P<0.05) and between male and female sheep (P<0.01). Serum leptin concentration was positively correlated with carcass fatness and there was a clear relationship between mean progeny serum leptin concentrations and the breeding value for fat. Serum leptin concentrations were greater (P<0.05) in lambs selected from the fat line (2.69ng/ml) than in lambs selected from the lean line (2.18ng/ml). A microsatellite marker, OarCP26, already mapped on sheep chromosome 4 (OAR4) was located in a sheep yeast artificial chromosome (YAC) clone that was positive for the leptin gene (LEP). This confirmed the independent assignment of LEP, mapped using a sheep cell hybrid panel, to OAR4. No significant differences in the allele frequencies of OarCP26 and two other flanking markers (TGLA116 and OarHH335) were detected in the lean and fat selection lines suggesting genes other than leptin are causing the differences in fat between the lean and fat Coopworth lines. },
	issn = {1176-5283},
	author = {Campbell AW and Broad TE and Jopson NB and Burkin HR and Dodds KG and Veenvliet BA and Bain WE}
}
@conference {41172,
	title = {Search for a locus near to myostatin that increases muscling in Texel sheep in New Zealand},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {60},
	year = {2000},
	month = {Jan},
	pages = {110-112},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	address = {Hamilton},
	abstract = {In close collaboration with three Texel breeders, we initially genotyped 36 rams with 5 DNA microsatellite markers that are known to map around the myostatin gene in sheep. Of these 36 rams, progeny of 12 polymorphic sires (4 from each flock) were selected for further analysis. This selection was made on the basis of each sire producing more than 40 offspring which were accompanied by parentage records, live weights at weaning and 8 months of age, and ultrasonic measurements of fat depth and rib-eye muscle dimensions. Final progeny numbers from the sires chosen ranged from 45 to 115 per sire. The data was analyzed by regressing the observed measurements on the probability of which paternal grandparental chromosome was inherited at the myostatin locus. The results suggest that the region of the sheep genome near the myostatin locus affects muscling (P<0.05) and fat depth (P<0.05) in the progeny of 4 of 12 sires tested. },
	issn = {1176-5283},
	author = {Broad TE and Glass BC and Greer GJ and Robertson TM and Bain WE and Lord EA and McEwan JC}
}
@conference {41141,
	title = {Mouse 3T3-L1 Fibroblast Cells as a Bioassay For Serum-Borne Adipogenic Factors},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {59},
	year = {1999},
	month = {Jan},
	pages = {279-281},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	abstract = {This study of the development of adipose tissue in sheep set out to investigate whether culture medium containing serum from Coopworth lean and fat selection lines had differential effects on proliferation and differentiation of mouse 3T3-L1 preadipocytes. Using fetal calf serum (FCS) as a positive control, the effects of serum from lean and fat lines of sheep and from goat and horse were compared by analysing total cell numbers, and protein and triglyceride (TG) levels per unit DNA in the mouse cell cultures. Differentiation was induced in all serum treatments. Mean cell numbers in cultures up to 13 days after differentiation were higher for sheep serum than for the other species (P<0.001). There was no significant difference (P>0.05) in protein content between FCS and serum from lean and fat sheep lines. Mean TG levels were significantly higher (P<0.001) for FCS than for the lean and fat selection lines, which did not differ significantly from each other (P>0.05). Significant differences in either proliferation or differentiation were not detected between the fat and lean sheep. },
	issn = {1176-5283},
	author = {Veenvliet BA and Littlejohn RP and Suttie JM and Broad TE}
}
@conference {40287,
	title = {Sheep map: The New Zealand programme to map the sheep genome},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {51},
	year = {1991},
	month = {Jan},
	pages = {85-86},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	abstract = {A series of four trials, each involving 1200 ewes have been  conducted over the period 1986 to 1990. Ewes have been treated with  various synchronisation treatments and the time to onset of oestrus  monitored each month of the year. Results indicate that while overall  mean values for each year don{\textquoteright}t differ, there were significant seasonal  effects within years with the interval to onset being shorter during the  breeding season. However, the maximum difference between monthly means  in any one year varied from 27 h to 17 h. Significant effects of  synchronisation device were seen with ewes treated with sponges having a  longer (+ 8 h) interval to onset than ewes treated with CIDR devices.  While effects of ewe age and ewe liveweight were seen in some trials no  consistent trend was identified. On most occasions significant  interactions with month of year indicated inconsistency of effect within  any one trial. The recorded variation in interval from device removal  to onset of oestrus is in agreement with that reported between flock in  the field. This variation is sufficient to effect conception rates to  artificial insemination performed on a time basis and evaluation of  contributing factors is required for the development of protocols for  this activity.  },
	issn = {1176-5283},
	author = {Hill DF and Broad TE}
}
