@conference {40276,
	title = {A view of recent development on the effect of nutrition on ovulation rate (the flushing effect) with particular reference to research at Ruakura},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {51},
	year = {1991},
	month = {Jan},
	pages = {15-24},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	abstract = {The use of unfrozen, diluted bovine semen for in vitro  fertilisation (IVF) was investigated. In vitro matured bovine  oocytes were incubated with various (0.125 - 4.0 x 10 6/ml)  concentrations of washed sperm. Penetration rates ranged from 21\% at  low sperm concentrations to 80-100\% above 1 x 10 6 sperm/ml (P<0.01).  Unfrozen sperm gave better penetration rates than frozen-thawed sperm.  Oocytes were incubated with washed sperm (1 x 10 6/ml) in increasing  dilutions of caprogen extender. Sperm penetration was significantly  inhibited at dilutions of 10-fold or less (P<0.01). Oocytes were  inseminated with washed sperm (1 x 10 6/ml) at various concentrations  (0.50 ug/ml) of heparin. No significant differences were found.  },
	issn = {1176-5283},
	author = {Smith JF}
}
