@conference {40621,
	title = {Anti goat IGF binding protein 3 antibodies from chicken egg yolk},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {54},
	year = {1994},
	month = {Jan},
	pages = {297-300},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	abstract = {The use of chickens for the production of highly specific antibodies  
to mammalian proteins offers a number of advantages over more conventional  
methods.  Antibody responses to IGFBP-3 immunogen were apparent in the  
serum of chickens after only two inoculations with low levels of the  
immunogen.  These specific antibodies subsequently appeared in the chicken  
egg yolk at levels of approximately 40\% of those estimated in serum. 
 
A simple, rapid and reproducible two step method for the extraction and  
purification of the immunoglobulin fraction from egg yolk using octanoic acid  
is described.  Relatively large quantities of specific antibodies can be  
obtained by these procedures.  Egg yolk antibodies generated in this study  
will be used to develop an ELISA for measuring levels of IGFBP-3 in plasma  
and other body fluids.  This approach is designed to overcome some of the  
difficulties associated with the use of ligand blotting analysis to quantify  
IGFBPs. },
	issn = {1176-5283},
	author = {McLaren RD and Prosser CG}
}
