@conference {40703,
	title = {Metabolic changes of cadmium and zinc in kidney and liver tissue during foetal development in Romney and Merino sheep},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {55},
	year = {1995},
	month = {Jan},
	pages = {176-178},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	abstract = {Diseases affecting mucosal surfaces, especially gastrointestinal  disorders, cause high mortality rates in young domestic livestock.   Hyperimmune milk products, using milk secretions from ruminants  immunised systemically with enteric pathogens, have been shown to confer  passive immunity against such disorders but the results are variable. To  study the type and titre of immunoglobulin (Ig) classes produced in milk  secretions after immunisation with gut pathogens, we have developed  ELISAs for the estimation of ovine IgG and IgA antibodies directed against  E. coli. Utilising an indirect ELISAs technique, the method involved  coating antigen onto microtitre plates then sequential addition of test sample  dilutions (containing primary antibody), anti-class-specific antibody and anti-  species horse radish peroxidase labelled antibody. Colorimetric end point  detection employed 3, 3},
	issn = {1176-5283},
	author = {Rounce JR and Lee J and Treloar BP and Grace ND and Sinclair BR}
}
