@conference {40776,
	title = {Utilisation of methionine by the mammary gland of the lactating goat},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {56},
	year = {1996},
	month = {Jan},
	pages = {53-57},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	abstract = {The goat milk protein, a-lactalbumin, contains no methionine residues,  hence if 35S label is supplied to the gland in the form of labelled methionine  only, the appearance of 35S in a-lactalbumin gives a measure of  transsulphuration of methionine to cysteine within the gland. Two lactating  Saanen goats were surgically modified with catheters implanted in the  mammary milk vein, femoral and external pudic mammary artery, and  jugular vein to enable infusion of 35S-methionine, into either the whole  body (jugular) or directly into the gland, and repetitive blood sampling.  Specific radioactivity of methionine in the blood and milk proteins was  determined at hourly intervals for 9 hours. Radioactivity of 35S was  determined in a-lactalbumin when 35S-methionine was supplied directly to  the gland via the mammary artery, but could not be detected when supplied  via the jugular vein. Analysis of mammary tissue from the latter experiment  did however show the appearance of 35S label in the intracellular cysteine  pool which was derived from methionine. Both approaches suggested that  transsulphuration within the gland, although small, may be important  especially when supply of methionine to the gland is high. The fractional extraction of plasma methionine by the gland for two goats  was 46.4},
	issn = {1176-5283},
	author = {Lee J and Treloar BP and Sinclair BR and Prosser CG and Davis SR and Harris PM}
}
